describe

The Q Exactive HF instrument uses a segmented quadrupole mass filter to better distinguish between analytes and co eluted compounds, achieving more accurate separation of parent ions. The advanced active ion beam transport component enhances the ion transport capability. The results have excellent sensitivity and a wider quantitative dynamic range, especially when dealing with complex mixtures.

The Q Exactive HF mass spectrometer supports multiple quantitative methods, including Selective Ion Monitoring (SIM), Parallel Reaction Monitoring (PRM), and Data Independence Acquisition (DIA). Users can choose the technology that best matches their sample and information requirements.

• SIM provides a simple method for setting up ion monitoring with the highest selectivity and sensitivity. This mode is most suitable for quantifying minority compounds in moderately complex samples.

• PRM - Parallel reaction monitoring provides high selectivity and sensitivity for reliable target confirmation. In this mode, quantification is performed after collection to achieve greater throughput. The high-resolution and precise mass number Orbitrap mass spectra ensure reliable confirmation through library matching.

• DIA - Data independence collection utilizes qualitative confirmation to achieve large-scale target proteomics research. Its simple and universal collection settings do not require detailed sample knowledge before data collection. DIA has improved the reproducibility and completeness of data through complete quantitative data recording. This mode enables retrospective analysis of more analytes.

The Q Exactive HF system features a brand new ultra-high field Orbitrap mass analyzer, which provides higher resolution and faster scanning speed in a smaller size. Combined with high-quality Orbitrap mass spectra, the Q Exactive HF system achieves more reliable qualitative and quantitative analysis in a shorter amount of time

• The scanning speed reaches 18 Hz, resulting in a 50% increase in the number of identified compounds within a shorter gradient

• Resolution up to 240000 (FWHM)m/z200 ensures the most reliable identification

• Its spectral quality is significantly better than Q-TOF mass spectrometer, with higher protein coverage and more reliable quantitative results

• Polarity switching (when the resolution is set to 60000,<1 second per complete cycle) enables the analysis of more different compounds in a single analysis

When studying complete proteins or protein complexes, the Q Exactive HF system can be equipped with a complete protein mode option (applicable only on new instruments). This option enhances the analytical capability of complete proteins through complex ion beam control and simple pressure adjustment.